Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Dec;12(1):108-116.
doi: 10.1080/21655979.2020.1860479.

A genome-wide survey of interaction between rice and Magnaporthe oryzae via microarray analysis

Yanping Tan  1 , Xiaolin Yang  2 , Minghao Pei  1 , Xin Xu  1 , Chuntai Wang  1 , Xinqiong Liu  1
Affiliations

A genome-wide survey of interaction between rice and Magnaporthe oryzae via microarray analysis

Yanping Tan et al. Bioengineered. 2021 Dec.

Abstract

The main aim of the work is to study the regulation of gene expression in the interaction between rice and Magnaporthe oryzae by gene chip technology. In this study, we mainly focused on changes of gene expression at 24, 48, and 72 hours post-inoculation (hpi), through which we could conduct a more comprehensive analysis of rice blast-related genes in the process of infection. The results showed that the experimental groups contained 460, 1227, and 3937 significant differentially expressed genes at 24, 48, and 72 hpi, respectively. Furthermore, 115 significantly differentially expressed genes were identified in response to rice blast infection at all three time points. By annotating these 115 genes, they were divided into three categories: metabolic pathways, proteins or enzymes, and organelle components. As expected, many of these genes were known rice blast-related genes; however, we discovered new genes with high fold changes. Most of them encoded conserved hypothetical proteins, and some were hypothetically conserved genes. Our study may contribute to finding new resistance genes and understanding the mechanism of rice blast development.

Keywords: Magnaporthe oryzae; Rice; interaction; microarray.

PubMed Disclaimer

Conflict of interest statement

No potential conflict of interest was reported by the authors.

Figures

None
Graphical abstract
Figure 1.
Figure 1.
The expression level of the genes chosen for confirmation by QRT-PCR at three time points
Figure 2.
Figure 2.
Trend analysis and annotation of the 115 significantly differentially expressed genes. (a) Using stem software to cluster the change trend of the 115 genes during the three time points. Marked color chart is a significant trend, such as the thirteenth and fifteenth diagram. (b) Three categories of the significant trend genes according to different functions by AgriGO database. C, F and P corresponding to cellular component, molecular function, and biological process, respectively. (c) KEGG analysis of the significant trend genes
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Gayen D, Paul S, Sarkar S, et al. Comparative nutritional compositions and proteomics analysis of transgenic Xa21 rice seeds compared to conventional rice. Food Chem. 2016;203:301–307. - PubMed
    1. Chen J, Lin S, Chen C, et al. Development of a microarray for two rice subspecies: characterization and validation of gene expression in rice tissues. BMC Res Notes. 2014;7:15–29. - PMC - PubMed
    1. Sarkar C, Saklani B, Singh P, et al. Variation in the LRR region of Pi54 protein alters its interaction with the AvrPi54 protein revealed by in silico analysis. Plos One. 2019;14(11):e0224088. - PMC - PubMed
    1. Zhao H, Wang X, Jia Y, et al. The rice blast resistance gene Ptr encodes an atypical protein required for broad-spectrum disease resistance. Nat Commun. 2018;9(1):2039. - PMC - PubMed
    1. Jones J, Dang J.. The plant immune system. Nature. 2006;444(7117):323–329. - PubMed

Publication types

Supplementary concepts

Grants and funding

This work was supported by the Fundamental Research Funds for the Central Universities South-Central University for Nationalities (CZY20035); the National Natural Science Foundation of China [31370306].
开云球赛中国有限公司 | 万搏官方网站 | c7官网登录入口 | 九州平台-九州(中国) | 万搏官方网站 | 千亿球友会·(中国)官方网站 | 米兰体育网址 | 开云手机官方网站 | 江南手机网页版登录入口 |