Loc680254 regulates Schwann cell proliferation through Psrc1 and Ska1 as a microRNA sponge following sciatic nerve injury

doi:10.1002/glia.24045

. 2021 Oct;69(10):2391-2403.

doi: 10.1002/glia.24045. Epub 2021 Jun 11.

Loc680254 regulates Schwann cell proliferation through Psrc1 and Ska1 as a microRNA sponge following sciatic nerve injury

Chun Yao¹, Qihui Wang¹, Yaxian Wang¹, Jiancheng Wu¹, Xuemin Cao¹, Yan Lu¹, Yanping Chen¹, Wei Feng¹, Xiaosong Gu^{1

2}, Xin-Peng Dun³, Bin Yu^{1

2}

Affiliations

¹ Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, China.
² Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair, Affiliated Hospital of Nantong University, Nantong University, Nantong, China.
³ Faculty of Medicine and Dentistry, Plymouth University, Plymouth, Devon, UK.

PMID: 34115425
DOI: 10.1002/glia.24045

Loc680254 regulates Schwann cell proliferation through Psrc1 and Ska1 as a microRNA sponge following sciatic nerve injury

Chun Yao et al. Glia. 2021 Oct.

. 2021 Oct;69(10):2391-2403.

doi: 10.1002/glia.24045. Epub 2021 Jun 11.

Authors

Chun Yao¹, Qihui Wang¹, Yaxian Wang¹, Jiancheng Wu¹, Xuemin Cao¹, Yan Lu¹, Yanping Chen¹, Wei Feng¹, Xiaosong Gu^{1

2}, Xin-Peng Dun³, Bin Yu^{1

2}

Affiliations

¹ Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, China.
² Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair, Affiliated Hospital of Nantong University, Nantong University, Nantong, China.
³ Faculty of Medicine and Dentistry, Plymouth University, Plymouth, Devon, UK.

PMID: 34115425
DOI: 10.1002/glia.24045

Abstract

Peripheral nerve injury triggers sequential phenotype alterations in Schwann cells, which are critical for axonal regeneration. Long noncoding RNAs (lncRNAs) are long transcripts without obvious coding potential. It has been reported that lncRNAs participate in diverse biological processes and diseases. However, the role of lncRNA in Schwann cells and peripheral nerve regeneration is unclear. Here, we identified an lncRNA, loc680254, which is upregulated in rat sciatic nerve after peripheral nerve injury. The loc680254 knockdown inhibits Schwann cell proliferation, enhances apoptosis, and hinders cell cycle, while loc680254 overexpression has the opposite effect. Mechanically, we found that loc680254 might act as a microRNA sponge to regulate the expression of mitosis-related gene, spindle and kinetochore associated complex subunit 1 (Ska1) and proline/serine-rich coiled-coil 1 (Psrc1). Silencing of Psrc1 or Ska1 attenuates the effect of loc680254 overexpression on Schwann cell proliferation. Finally, we repaired the rat sciatic nerve gap with chitosan scaffolds loaded with loc680254-overexpressing Schwann cells and evaluated axon regeneration and functional recovery. Our results indicated that loc680254 is a new potential modulator for Schwann cell proliferation, which could be targeted to develop novel therapeutic strategies for peripheral nerve repair.

Keywords: Schwann cells; cell proliferation; competing endogenous RNA; lncRNA; peripheral nerve injury.

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References

REFERENCES

1. Agthong, S., Kaewsema, A., Tanomsridejchai, N., & Chentanez, V. (2006). Activation of MAPK ERK in peripheral nerve after injury. BMC Neuroscience, 7, 45. https://doi.org/10.1186/1471-2202-7-45
1. Arthur-Farraj, P. J., Latouche, M., Wilton, D. K., Quintes, S., Chabrol, E., Banerjee, A., Woodhoo, A., Jenkins, B., Rahman, M., Turmaine, M., Wicher, G. K., Mitter, R., Greensmith, L., Behrens, A., Raivich, G., Mirsky, R., & Jessen, K. R. (2012). C-Jun reprograms Schwann cells of injured nerves to generate a repair cell essential for regeneration. Neuron, 75(4), 633-647. https://doi.org/10.1016/j.neuron.2012.06.021
1. Bozkurt, A., Deumens, R., Scheffel, J., O'Dey, D. M., Weis, J., Joosten, E. A., Führmann, T., Brook, G. A., & Pallua, N. (2008). CatWalk gait analysis in assessment of functional recovery after sciatic nerve injury. Journal of Neuroscience Methods, 173(1), 91-98. https://doi.org/10.1016/j.jneumeth.2008.05.020
1. Cattin, A. L., & Lloyd, A. C. (2016). The multicellular complexity of peripheral nerve regeneration. Current Opinion in Neurobiology, 39, 38-46. https://doi.org/10.1016/j.conb.2016.04.005
1. Fontana, X., Hristova, M., Da Costa, C., Patodia, S., Thei, L., Makwana, M., Spencer-Dene, B., Latouche, M., Mirsky, R., Jessen, K. R., Klein, R., Raivich, G., & Behrens, A. (2012). C-Jun in Schwann cells promotes axonal regeneration and motoneuron survival via paracrine signaling. The Journal of Cell Biology, 198(1), 127-141. https://doi.org/10.1083/jcb.201205025

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[1] Agthong, S., Kaewsema, A., Tanomsridejchai, N., & Chentanez, V. (2006). Activation of MAPK ERK in peripheral nerve after injury. BMC Neuroscience, 7, 45. https://doi.org/10.1186/1471-2202-7-45

[2] Agthong, S., Kaewsema, A., Tanomsridejchai, N., & Chentanez, V. (2006). Activation of MAPK ERK in peripheral nerve after injury. BMC Neuroscience, 7, 45. https://doi.org/10.1186/1471-2202-7-45

[3] Arthur-Farraj, P. J., Latouche, M., Wilton, D. K., Quintes, S., Chabrol, E., Banerjee, A., Woodhoo, A., Jenkins, B., Rahman, M., Turmaine, M., Wicher, G. K., Mitter, R., Greensmith, L., Behrens, A., Raivich, G., Mirsky, R., & Jessen, K. R. (2012). C-Jun reprograms Schwann cells of injured nerves to generate a repair cell essential for regeneration. Neuron, 75(4), 633-647. https://doi.org/10.1016/j.neuron.2012.06.021

[4] Arthur-Farraj, P. J., Latouche, M., Wilton, D. K., Quintes, S., Chabrol, E., Banerjee, A., Woodhoo, A., Jenkins, B., Rahman, M., Turmaine, M., Wicher, G. K., Mitter, R., Greensmith, L., Behrens, A., Raivich, G., Mirsky, R., & Jessen, K. R. (2012). C-Jun reprograms Schwann cells of injured nerves to generate a repair cell essential for regeneration. Neuron, 75(4), 633-647. https://doi.org/10.1016/j.neuron.2012.06.021

[5] Bozkurt, A., Deumens, R., Scheffel, J., O'Dey, D. M., Weis, J., Joosten, E. A., Führmann, T., Brook, G. A., & Pallua, N. (2008). CatWalk gait analysis in assessment of functional recovery after sciatic nerve injury. Journal of Neuroscience Methods, 173(1), 91-98. https://doi.org/10.1016/j.jneumeth.2008.05.020

[6] Bozkurt, A., Deumens, R., Scheffel, J., O'Dey, D. M., Weis, J., Joosten, E. A., Führmann, T., Brook, G. A., & Pallua, N. (2008). CatWalk gait analysis in assessment of functional recovery after sciatic nerve injury. Journal of Neuroscience Methods, 173(1), 91-98. https://doi.org/10.1016/j.jneumeth.2008.05.020

[7] Cattin, A. L., & Lloyd, A. C. (2016). The multicellular complexity of peripheral nerve regeneration. Current Opinion in Neurobiology, 39, 38-46. https://doi.org/10.1016/j.conb.2016.04.005

[8] Cattin, A. L., & Lloyd, A. C. (2016). The multicellular complexity of peripheral nerve regeneration. Current Opinion in Neurobiology, 39, 38-46. https://doi.org/10.1016/j.conb.2016.04.005

[9] Fontana, X., Hristova, M., Da Costa, C., Patodia, S., Thei, L., Makwana, M., Spencer-Dene, B., Latouche, M., Mirsky, R., Jessen, K. R., Klein, R., Raivich, G., & Behrens, A. (2012). C-Jun in Schwann cells promotes axonal regeneration and motoneuron survival via paracrine signaling. The Journal of Cell Biology, 198(1), 127-141. https://doi.org/10.1083/jcb.201205025

[10] Fontana, X., Hristova, M., Da Costa, C., Patodia, S., Thei, L., Makwana, M., Spencer-Dene, B., Latouche, M., Mirsky, R., Jessen, K. R., Klein, R., Raivich, G., & Behrens, A. (2012). C-Jun in Schwann cells promotes axonal regeneration and motoneuron survival via paracrine signaling. The Journal of Cell Biology, 198(1), 127-141. https://doi.org/10.1083/jcb.201205025

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Loc680254 regulates Schwann cell proliferation through Psrc1 and Ska1 as a microRNA sponge following sciatic nerve injury

Affiliations

Loc680254 regulates Schwann cell proliferation through Psrc1 and Ska1 as a microRNA sponge following sciatic nerve injury

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